Mechanism of CSF outflow through human arachnoid granulations using in-vitro and ex-vivo perfusion models
نویسندگان
چکیده
منابع مشابه
Ex vivo model of cerebrospinal fluid outflow across human arachnoid granulations.
PURPOSE The brain's arachnoid membrane with granulations is an important biological barrier whose responsibilities include the transmission of cerebrospinal fluid (CSF) and the regulation of pressure. Membrane disturbance may cause changes that are difficult to replicate with animal models, suggesting the need for a model using human arachnoid membrane with granulations for the study of conditi...
متن کاملIn vitro model of cerebrospinal fluid outflow through human arachnoid granulations.
PURPOSE To describe and validate an in vitro model of the arachnoid granulation (AG) outflow pathway for cerebrospinal fluid (CSF), by using human AG cells grown on a filter membrane support and perfused in a modified Ussing chamber at pressures analogous to normal human intracranial pressures. METHODS Human AG cells were grown, characterized, seeded onto filter membranes, and perfused in the...
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There has been no general agreement regarding the presence of arachnoid granidations and arachnoid villi in mammals. This seems to have arisen from the fact that various workers have used different criteria in distinguishing between the two terms — 'granula tion' and 'villus'. Luschka (1852) was the first to. define the structure observed macroscopically as an 'arachnoid granulation', while fo...
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The arachnoid membrane (AM) and granulations (AGs) are important in cerebrospinal fluid (CSF) homeostasis, regulating intracranial pressure in health and disease. We offer a functional perspective of the human AM's transport mechanism to clarify the role of AM in the movement of CSF and metabolites. Using cultures of human AG cells and a specialized perfusion system, we have shown that this in ...
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ژورنال
عنوان ژورنال: Cerebrospinal Fluid Research
سال: 2006
ISSN: 1743-8454
DOI: 10.1186/1743-8454-3-s1-s11